A major thrust of this research will focus on the use of new model grasses as drivers for lignocellulosic feedstock development and
The genomes of both species have recently been sequenced and transformation technologies have been developed for each. Members of the Brutnell lab have contributed to the development of transformation technologies for Setaria viridis and have helped annotate the S. viridis and B. distachyon genomes. They are currently developing genetic resources for both systems that include chemically- and radiation- mutagenized populations. They are also tapping high-throughput sequencing techniques to accelerate reverse and forward genetic screens and to develop a gene atlas for S. viridis using RNAseq technology.
Another important component of their research program is the development of reverse genetics tools for gene discovery in maize. Over the past ten years, members of the Brutnell lab have been mobilizing and mapping the maize transposable elements Ac and Ds insertions throughout the maize genome. They have also been developing genetic and molecular protocols for using Ac and Ds as insertional mutagens. These elements tend to insert at closely linked sites in the genome to create unstable genetic variation. They have shown how these transposons can be used to fine map gene structure and have several targeted mutagenesis programs underway.
Finally, a fundamental biological challenge that the Brutnell lab is trying to address is to understand the mechanisms that drive C4 photosynthetic differentiation. Using the forward and reverse genetics resources they have developed for S. viridis and Z. mays, they are now generating mutants in the genes necessary for the C4 carbon shuttle and using a combination of informatics and molecular approaches to define the regulatory networks driving their expression. These studies are targeted at understanding the function of genes involved in C4 photosynthesis and have applications in breeding/engineering improved photosynthetic traits in C4 grasses and in introducing novel C4 traits into C3 grasses.
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CURRENT GRANTS AND FUNDING AGENCIES
- A Systems Approach to the Development and Function of C4 Photosynthesis, National Science Foundation, Award #IOS-1127017
- Defining Determinants and Dynamics of Cellulose Microfibril Biosynthesis, Assembly
andDegradation, U. S. Department of Energy, Award #DE-SC0006645
- Genome-wide Impact of mPing Transposition on Rice Phenotypic Diversity, National Science Foundation, Award #IOS-1027542
- A Systems-Level Analysis of Drought and Density Response in the Model C4 Grass Setaria viridis, U. S. Department of Energy, Award #DE-SC0008769
- Biosystems Design Awards-Genomic Science brochure
- Engineering the Symbiosis Signaling Pathway of Cereals for Recognition of Nitrogen Fixing Bacteria, The Bill & Melinda Gates Foundation, Award #OPPI028264
- Ds Regional Mutagenesis: a Platform for Defining Gene Function in Maize, National Science Foundation, Award #IOS-1314143
- Translational Dynamics of Leaf and Chloroplast Development in Maize, National Science Foundation, Award #IOS-1339130
- Systems Analysis of the Physiological and Molecular Mechanisms ofSorghum Nitrogen Use Efficiency, Water Use Efficiency and Interactions with the Soil Microbiome, U. S. Department of Energy, Award #DE-SC0014395
- C4-Rice Phase 3, The Bill & Melinda Gates Foundation, Award #OPP1129902
- Engineering C4 photosynthesis in maize to enhance nitrogen utilization, U. S. Department of Agriculture, National Institute of Food and Agriculture, Award #2016-67013-24585
- Dissecting the genetic networks underlying Kranz anatomy in C4 grasses, National Science Foundation, Award #1546882