SETARIA

Setaria as a model system                                        

Setaria viridis is a C4 Panicoid grass with many favorable attributes for a model C4 genetic system including short stature, rapid lifecycle, high seed production and simple growth requirement (read the article here). We are currently developing numerous resources to accelerate the adoption of this model plant.

Click here for a list of abstracts from the 2nd International Setaria Genetics Conference.

Setaria for biofuels

 

The objectives of this project are to utilize genomic, computational, and engineering tools to begin the genetic dissection of drought and density response in S. viridis.

This project is funded by the United States Department of Energy’s Biological and Environmental Research Program.

Learn more about Setaria for Biofuels at Foxmillet

Setaria methods & protocols                                        

We have developed a methodology for performing crosses in Setaria viridis (S. viridis). The method involves pruning the panicle prior to a hot water treatment to kill viable pollen.

Crosses are performed following a well-controlled growth regime and typically result in the recovery of 1 to 7 cross-pollinated seed/s per panicle.

 

Setaria mutagenesis

 

We have generated and characterized approximately 3000 NMU mutant families useful for forward and reverse genetics in Setaria.  From these NMU mutagenized families, a TILLING population is also being developed which will be screened through a high throughput Next-Gen sequencing pipeline. 

Setaria recombinant inbred line development

We are also constructing six recombinant inbred populations generated by crossing diverse S. viridis accessions with A10.1. We have assembled a diverse germplasm collection of 390 S. viridis accessions with contributions from many collaborators. These accessions are being resequenced at JGI-DOE to establish a panel for population genetic analysis and genome-wide association studies (GWAS).

We demonstrated that phenotypes of interest can be mapped to fine resolution with a thorough characterization of the standing variation in a subset of this panel.  A subset of these 390 lines has been characterized for phenotypic traits and has been propagated for seed distribution at the USDA GRIN.

– To order the high transformation efficiency line ME034V:  ARS-GRIN

– To order the sequenced reference line A10.1:  ARS-GRIN

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