Setaria as a model system
Setaria viridis is a C4 Panicoid grass with many favorable attributes for a model C4 genetic system including short stature, rapid lifecycle, high seed production and simple growth requirement (read the article here). We are currently developing numerous resources to accelerate the adoption of this model plant.
Click here for a list of abstracts from the 2nd International Setaria Genetics Conference.
Setaria for biofuels
The objectives of this project are to utilize genomic, computational, and engineering tools to begin the genetic dissection of drought and density response in S.
This project is funded by the United States Department of Energy’s Biological and Environmental Research Program.
Learn more about Setaria for Biofuels at Foxmillet.
Setaria methods & protocols
- Protocol for Growing Setaria
- Wet moss treatment method for breaking seed dormancy
- How to perform crosses in Setaria
We have developed a methodology for performing crosses in Setaria viridis (S.
Crosses are performed following a well-controlled growth regime and typically result in the recovery of 1 to 7 cross-pollinated seed/s per panicle.
We have generated and characterized approximately 3000 NMU mutant families useful for forward and reverse genetics in Setaria. From these NMU mutagenized families, a TILLING population is also being developed which will be screened through a high throughput Next-Gen sequencing pipeline.
Setaria recombinant inbred line development
We are also constructing six recombinant inbred populations generated by crossing diverse S.
We demonstrated that phenotypes of interest can be mapped to fine resolution with a thorough characterization of the standing variation in a subset of this panel. A subset of these 390 lines has been characterized
– To order the high transformation efficiency line ME034V: ARS-GRIN
– To order the sequenced reference line A10.1: ARS-GRIN